凤凰城线上娱乐:Gateway? Technology

Gateway®克隆技術的基礎是 λ噬菌體的位點特異性重組反應,一種保存遺傳信息的有效的生化途徑(見圖1)。與傳統的需要DNA限制性內切酶、DNA連接酶、凝膠電泳和DNA片段純化等多個步驟的繁瑣亞克隆方法相比,該方法只需一步生化反應而且操作方便、快捷(室溫60分鐘),省時高效(見圖2)。另外,根據目的蛋白的生產和純化的不同要求, Invitrogen公司提供一系列含有不同啟動子和融合標簽、以及在幾種宿主表達的目標載體。有關Gateway®克隆技術的詳細信息,請訪問Invitrogen公司的網址:www.invitrogen.com。

 

圖1. λ噬菌體的位點特異性重組反應。(點擊放大) 圖2. 一步法從ORFEXPRESS™ 穿梭克隆轉移ORF到目的載體

 

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The purchase of ORFEXPRESS™ Gateway® Shuttle Clones is accompanied by a limited label license under U.S. Patents 5,888,732; 6,143,557; 6,171,861; 6,270,969; 6,277,608 owned by Invitrogen, Corp. only to use the product for the internal research purposes of the purchaser. For information on purchasing a license to use the purchased product for purposes other than the internal research of the purchaser, contact the Director of Licensing at Invitrogen, Corp., 1600 Faraday Avenue, Carlsbad, CA 92008.

The trademark Gateway® is owned by Invitrogen Corporation.